Measuring Gene Expression : The Basics

Contents: Abbreviations. Preface. 1. Gene expression and its control. 2. Isolation and analysis of RNA. 3. Hybridization-based methods for measuring transcript levels. 4. PCR-based methods for measuring transcript levels. 5. Differential display, subtractive hybridization, amplification suppression and SAGE techniques for measuring gene expression. 6. Measuring gene expression using reporter gene assays. 7. Analysis of the proteome. 8. Statistical analysis of gene expression data. Index.

"In the post-genomic age, much biomedical research looks at when, where and at what level genes are expressed. Measuring Gene Expression is an all-in-one introduction to the main methods of measuring gene expression, including PCR - pased methods, differential display, reporter gene assays, microarrays, and proteomics, as well as a section on RNA isolation and analysis. It provides the information and protocols required to allow the reader to select the most appropriate method to measure gene expression in their experimental system and then interpret the results."