PCR : The Basics

Contents: Abbreviations. Preface. 1. An introduction to PCR. 2. Understanding PCR. 3. Reagents and instrumentation. 4. Optimization of PCR. 5. Analysis, sequencing and in vitro expression of PCR products. 6. Purification and cloning of PCR products. 7. PCR mutagenesis. 8. Analysis of gene expression. 9. Real-time RT-PCR. 10. Cloning genes by PCR. 11. Genome analysis.

"As in the first edition, the concept underlying this new edition of PCR is to provide an introductory text that will be useful to students and scientists who want to understand and use PCR for experimental purposes. The fundamental principles of performing PCR and analyzing and cloning products are fully explained, allowing the reader to understand the basics and to adapt PCR for specialized techniques. A select number of PCR applications, including real-time PCR, are discussed in detail, PCR, second edition, provides the information and protocols required to allow the reader to start using PCR, either simply as a routine tool, or as a starting point for developing new and innovative processes."