Plant Biochemistry: Techniques and Procedures

Contents: Part I: Analytical Techniques. 1. Introduction. 2. Chromatography. 3. Paper chromatography. 4. Column chromatography. 5. Thin layer chromatography. 6. Electrophoresis. 7. Ion exchange chromatography. 8. Gel-filtration chromatography. 9. Solvent extraction. 10. Dialysis, ultra filtration and lyophilisation.11. Centrifugation.12. Gas liquid chromatography. 13. High pressure liquid chromatography. 14. GC/MS and LC/MS. 15. Spectrophotometry. 16. Infra red spectroscopy. 17. Flame photometry. 18. Atomic-absorption spectroscopy. 19. Inductively coupled plasma atomic emission spectroscopy. 20. NMR Spectroscopy. 21. Radiochemical methods. 22. Mass spectrometry. 23. Polarimetry. 24. Conductimetry. 25. Potentiometry. 26. ELISA and radioimmunoassay (RIA). 27. Gravimetric analysis.  28. Volumetric analysis. 29. Amplification and sequencing of nucleic acids. Part II: Analytical Methods and Procedures: 28. Methods of analysis. 29. Carbohydrates and their methods of analysis. 30. Fats/oils/lipophilic constituents. 31. Amino acids and proteins. 32. Enzymes. 33. Nucleic acids. 34. Vitamines. 35. Phenolics and antinutrients. 36. Toxins. 37. Alkaloids. 38. Plant nutrients. 39. Plant pigments. 40. Pesticide residue analysis.

This book is aimed at helping the analyst with respect to analysis of samples, mainly, plant material. The book is divided into two parts. The first part deals with the different techniques involved in the biochemical analysis. The theoretical aspects of the same are presented in detail.  The mechanism and some information on the buildup of the equipments are furnished.  The need and scope of the instruments and their utility are also presented. The second part deals with specific methods of analysis. Analytical methods of sugars, amino acids, proteins, fats, enzymes and their components are presented.  Nucleic acid, vitamin, antinutrient (toxicants) and mineral analytical methods also have been included.  Information on alkaloid and pesticide residue analytical methods etc. has also been furnished. In many cases two or more methods have been given. They have been given in such a way that they include reagents, apparatus and step wise procedures. Final calculations needed for arriving at the concentration of the constituents have also been included.