Practical Biochemistry : Laboratory Mannual for Pharmacy Students

Contents: 1. General Rules. 2. Terms in common use for Biochemical work. 3. Colorimetry: spectrophotometer. Qualitative Analysis. 4. General reaction of carbohydrates. 5. Scheme for the identification of carbohydrates. 6. General reactions of proteins and amino acids. 7. Scheme for the identification of proteins. 8. General reactions of lipids. 9. Salivary amylase-mode of action. 10. Immobilization of enzyme. 11. Urine analysis. 12. Analysis of normal urine. 13. Analysis for abnormal constituents in urine. 14. Scheme for the identification of biologically important substances. 15. Paper electrophoresis. 16. Titration curve for amino acids. Quantitative analysis. 17. Collection of specimens for analysis. 18. Estimation of DNA. 19. Estimation of RNA. 20. Estimation of glucose. 21. Estimation of glycogen. 22. Estimation of serum cholesterol. 23. Estimation of blood sugar. 24. Estimation of serum  Protein. 25. Estimation of  Blood urea. 26. Estimation of serum Bilirubin. 27. Estimation of serum uric acid. 28. Estimation of serum creatinine. 29. Estimation of urine creatinine. 30. Estimation of urinary chloride. 31. Estimation of serum alkaline phosphatase activity. 32. Estimation of serum acid phosphatase activity. 33. Estimation of serum glutamate pyruvate transaminase (SGPT) Activity. 34. Estimation of serum glutamate Oxaloacetate transaminase (SGOT) Activity.

This manual is written with an objective to provide brief, complete and easily understandable various aspects of Biochemistry for Pharmacy students. Scheme for the identification of carbohydrates, lipids, proteins and biologically important compounds is given so that students can themselves perform experiments by following the said procedures. The chapter on the preparation of various types of solutions, buffer, standard curve and the terms which are used frequently in the biochemical work, have been explained with suitable examples and in a very simple, easily understandable manner. The chapter on immobilization of enzyme is illustrated in a very simple way and the students can get aware with the immobilization techniques by performing very simple exercises. For quantitative analysis in every experiment, complete procedure in tabular form and also calculations have been mentioned so that students can prepare standard curve themselves without any problem. The space has also been provided to record the readings and other data. At the end of the manual as well as in each chapter, important points are highlighted separately.