Practical Manual of Veterinary Biochemistry

Contents: Preface. 1. System international (SI) units. 2. Standardization of acids and Alkalis. 3. Titration curve of acid versus base. 4. Preparation of buffers and determination of pH. 5. Reaction of mono-Di and polysaccharides and their identification. 6. Estimation of lactose in milk. 7. Determination of acid number of oil/fat. 8. Determination of proteins. 9. Estimation of Amino acids (Sorensen’s method). 10. Anticoagulants used for blood analysis. 11. Colorimetry. 12. Flame photometry. 13. Estimation of serum total protein. 14. Estimation of blood glucose. 15. Glucose tolerance test. 16. Estimation of cholesterol. 17. Estimation of urea in serum. 18. Estimation of serum Bilirubin. 19. Urine analysis. 20. Examination of normal urine. 21. Analysis of normal urine. 22. Examination of pathological urine. 23. Analysis of pathological urine. 24. Estimation of glucose in urine (Benedict’s method). 25. Determination of protein in urine (Esbach’s method). 26. Determining the effects of pH and temperature on enzyme activity. 27. Paper chromatography. 28. Electrophoretic separation of peoteins. 29. Analysis of proteins using HPLC. 30. Isolation of protein using affinity chromatography. 31. Immunocytochemical localization of a peptide/protein. 32. Animal cell culture. 33. Blotting methods. 34. Nucleic acid probe preparation and hybridization. 35. Estimation of enzymes. 36. Clinical examination of cerebrospinal fluid. 37. Clinical examination of semen. 38. DNA and RNA extraction. 39. Polymerase chain reaction (PCR). 40. Autoradiographic detection of specific biomolecules. 41. Cell culture experiments using radio labelled precursors and liquid scintillation counting. 42. Demonstration of hybridoma technique. 43. Embryo sexing by PCR. 44. Mirrochondrial DNA and PCR in farm animals. 45. Food analysis. 46. Analysis of food toxins. 47. Immunochemical assay. 48. Induction of recombinant protein. 49. Estimation of protein by Lowry’s method. 50. References. 51. Index.

Practical Manual of Veterinary Biochemistry, designed to cater to the requirements of students of Veterinary Biochemistry, Microbiology, Physiology, Animal Genetics and Breeding etc., covers all the basic and applied techniques employed for qualitative and quantitative analysis of biomolecules. This book will give preliminary information to the beginners in the field of Biochemistry, molecular biology and biotechnology. This book provides concise information on practical aspects of the techniques employed in biochemical studies for the undergraduate and post graduate students, instructors and research workers.