Separation Processes in Biotechnology
Contents: Foreword/Alan S. Michaels. Preface. I. Separation processes: 1. Selection of operations in separation processes/Jaun A. Asenjo. 2. Chemical, physical and biochemical concepts in isolation and purification of proteins/Milton T.W. Hearn and Birger Anspach. II. Product release and recovery: 3. Protein secretion systems in microbial and Mammalian cells/Donald T. Moir and Jen-I Mao. 4. Cell disruption by Homogenizer/Cady R. Engler. 5. Bead Mill disruption/Horst Schutte and Maria-Regina Kula. 6. Enzymatic cell Lysis for product release/Juan A. Asenjo and Barbara A. Andrews. 7. Chemical permeabilization of cells for intracellular product release/Thomas J. Naglak, David J. Hettwer and Henry Y. Wang. III. Concentration and separation operations: 8. Membrane systems/A.G. Fane and J.M. Radovich. 9. Ion-exchange processes/Charles D. Shuey. 10. Aqueous two-phase separations/Per-Ake Albertsson, Gote Johansson, and Folke Tjerneld. 11. Precipitation/Charles E. Glatz. IV. Purification operations: 12. Ion exchange in purification/Nien-Hwa Linda Wang. 13. Process affinity chromatography/Yannis D. Clonis. 14. Reversed-phase and hydrophobic interaction chromatography of peptides and proteins/Ziad El Rassi, Ann L. Lee and Csaba Horvath. 15. Analysis of effluent profiles in large-scale liquid chromatography/J. Hong. 16. Electrically driven separation processes: analytical and preparative methods/Cornelius F. Ivory. V. Design of separation processes: 17. Synthesis of downstream processes/George J. Prokopakis and Juan A. Asenjo. 18. Automation in protein purification: the use of expert systems for control of process chromatography/Andrew C. Kenny. 19. Downstream processing plant and equipment/M.P. Brocklebank. 20. Downstream process economics/Rajiv Datar and Carl-Gustaf Rosen. Index.
"This book provides comprehensive details on the advances in recovery, separation and purification operations of bioprocess technology.
Written by over 30 international experts, Separation Process in Biotechnology contains practical information on topics such as physical, chemical and biochemical characterization of products and their contaminants…recent advances in protein secretion systems, differential product release, and membrane permeabilization…separation and concentration operations including ion-exchange, membrane processes, aqueous two-phase extractions, and precipitation…purification operations including affinity chromatography, reversed-phase chromatography, hydrophobic interaction chromatography, ion exchange purification, and electrically driven separations…computer methods in chemical engineering and recent developments in artificial intelligence, and automation in chromatography… and more.
Encouraging communication between biological scientists and chemical engineers, Separation Processes in Biotechnology is a useful reference for biochemical, chemical and process engineers; biotechnologists; protein, applied and industrial chemists and biochemists; industrial microbiologists; and food technologists."
