Working with DNA: The Basics

Contents: Preface. 1. Understanding recombinant DNA techniques. 2. The recombinant DNA laboratory. 3. Solutions, buffers, stocks and cocktails. 4. Cloning vectors. 5. Restriction endonucleases. 6. Polymerases. 7. The polymerase chain reaction. 8. Working with DNA: the reasons. Index.

"Working with DNA: The Basics is a guide to the materials and methods that are used in the recombinant DNA laboratory. It is designed to help the novice understand how specific laboratory techniques work, and also has sections that may stimulate the thinking of experienced researchers. It isn\'t a book of step by step protocols and recipes; it is intended to broaden and strengthen the foundation of the readers, so that working with DNA becomes more natural and they are more aware of the many ways that a molecular research question may be addressed."