Agricultural Biotechnology

Contents: 1. Lab Safety Precautions. 2. Basic Lab Instruments. 3. Methods of Regents Preparations. 4. Extraction of Deoxyribose Nucleic Acid (DNA) from Plant Leaf Tissue. 5. Quantification of DNA. 6. Polymerase Chain Reaction for DNA Amplification. 7. To Identify Importance of Morphological, Physiological and Yield Traits of Ideal Crop Plants. 8. To Study Different Types of Agricultural Tools Required for Experimental Layout in the Field. 9. To Perform Field Layout for Crop Experiment. 10. Chemicals and Reagents.

The science of agriculture is among the oldest and most intensely studied by mankind. Human intervention has led to manipulation of plant gene structure for the use of plants for the production of bio-energy, food, textiles, among other industrial uses. A sound knowledge of biotechnology and its implementation in farmer’s field through research intervention is required. This manual will provide an overview of all these aspects along with an updated review of the major plant biotechnology procedures and techniques, their impact on novel agricultural development, and crop plant improvement. This book integrates basic biotechnological methodologies along with different tools, layouts and designs of field experiments. This manual spreads overview to up-to-date agricultural practices, offering solutions to specific agricultural needs and problems from plant and crop yield. It presents and evaluates the limitations of classical methodologies and the potential of novel and emergent agriculturally related biotechnologies. The manual provides an holistic view that makes it a valuable source of information for researchers of agriculture and biotechnology as well as microbiologists, agricultural engineers, environmental biologists, environmental engineers, and environmentalists. Short exercises at the end of the chapters help to make the book suitable for course work in agricultural biotechnology, genetics, biology, biotechnology and plant science courses.