Animal Cell Culture and Virology
Contents: Preface. 1. Introduction. 2. Principles of cell culture. 3. The cell and its environment. 4. Types of cells and cell culture. 5. Cell culture: applications, advantages and disadvantages. 6. Ideal cell culture laboratory with equipments. 7. Growth and maintenance of cells in culture. 8. Components of medium and its preparation. 9. Primary cell culture. 10. Maintenance of cell culture. 11. Packaging and transportation of cells. 12. Description of commonly used cell lines. 13. Subculturing of cells in different vessels. 14. Scaling up of animal cell culture. 15. Cell line authentication and characterization. 16. Cryopreservation and thawing/revival of cells. 17. Contamination of cell culture and curing. 18. Counting of cells. 19. Isolation and identification of virus in cell culture. 20. Virus assays. 21. Collection, preservation and transport of various specimens for laboratory diagnosis. 22. Collection of materials for diagnosis of viral diseases. 23. Processing of laboratory specimens, inoculation into laboratory animals and embryonated eggs for pathogen isolation. 24. Serum neutralization test. 25. Enzyme Linked Immunosorbent Assay (ELISA). 26. Biosafety measures and risk management. Glossary. Appendix. References.
"The book is written in a very simple and lucid manner so that everybody can read and understand it very easily. The book is useful for scientists, teachers, students, officers, diagnosticians and laboratory technicians as cell culture has become an essential and indispensable tool in many branches of life sciences and application of cell culture is getting increased exponentially day by day in various fields of biological and medical research arena. This book will provide detailed information on all aspects of the cell culture starting from establishment of a cell culture laboratory, primary culture, secondary culture, media filtration, collection, preservation and dispatch of samples for diagnosis of viral diseases, cell line authentication and characterization, contamination and curing, cryopreservation of cells and revival of cells besides description on ELISA, SNT, Virus Titration etc.
This book will be extremely useful to all persons who are directly or indirectly involved in cell culture work for various biological experiments. Finally, students and examinees can enrich their knowledge on cell culture from the book and can face any challenge easily and confidently."
