Laboratory Techniques of Basic Microbiology for the Beginners

Contents: Preface. Foreword. 1. Study of basic laboratory instructions and terms in microbiology laboratory. 2. Study of compound bright field microscope. 3. Study of microscopy. 4. Focusing and care of microscope. 5. Study of microscopic measurement micrometry. 6. Study of different types of microscopes. 7. Study of aids/tools apparatus devices of microbiology lab. 8. Cleaning and maintenance of glassware and plastic ware in microbiology lab. 9. Sterilization by dry heat. 10. Sterilization by moist heat. 11. Sterilization by filtration. 12. Sterilization by chemical agent. 13. Gaseous sterilization. 14. Sterilization by irradiation. 15. Study of laminar flow hood and aseptic technique. 16. Study of culture media. 17. Culture media preparation. 18. Adjustment of pH of a media. 19. Enumeration of bacteria, and study of colony characters. 20. Purification of mixed bacterial culture by streak plate method. 21. Inoculation of liquid media and study of colony characters. 22. Slant inoculation and preservation of isolates. 23. Inoculation by stab method. 24. Enumeration of bacteria of surfaces by swabbing. 25. Environmental conditions affecting bacterial growth. 26. Study of wet mount technique. 27. Motility test by hanging drop technique and culture method. 28. Study of stains, smear preparation and simple staining. 29. Negative staining. 30. Capsular staining. 31. Gram staining. 32. Spore staining. 33. Acid-fast staining. 34. Flagellar staining. 35. Biochemical tests for identification of bacteria. 36. Study and enumeration of coliforms, fecal coliforms and E. Coli. 37. Observation of fungi by slide culture technique. 38. Study of year morphology. 39. Study of algae. 40. Study of protozoa. 41. Study of bacteriophages. References.