Contents: Foreword. Preface. 1. Laboratory safety and precautions. 2. Isolation of genomic DNA. 3. Isolation of plasmid DNA. 4. Isolation of total RNA. 5. Quantification of nucleic acids. 6. Polymerase chain reaction. 7. Variants of polymerase chain reaction. 8. Reverse transcription polymerase chain reaction (RT-PCR). 9. Agarose gel electrophoresis. 10. Single strand conformation polymorphism (SSCP). 11. Restriction digestion. 12. Real time PCR. 13. Chromatin immunoprecipitation assay. 14. Microsatellite analysis. 15. DNA ligation competent cells preparation and transformation. 16. Retrieving QTL and SNP information from databases. 17. DNA Microarray. 18. In situ hybridization. 19. Preparation of required buffers and solutions.
