Techniques And Methods In Biology
Contents: Preface. Acknowledgements. Part I: Biophysical Methods: 1. Spectroscopy. 2. Infrared Spectroscopy. 3. Ultraviolet and Visible Spectroscopy. 4. Colorimetry and Visible Spectrophotometry. 5. Fluorescence and Phosphorescence Spectrophotometry. 6. Optical Rotatory Dispersion and Circular Dichroism. 7. Nuclear Magnetic Resonance Spectroscopy. 8. Electron Spin Resonance Spectroscopy. 9. Atomic Absorption Spectroscopy. 10. X-ray Crystallography/Diffraction. 11. Mass Spectrometry. 12. Chromatography. Part II: Microscopy: 13. Electron Microscopy. 14. Optical/light Microscopy. 15. Fixation and Staining. 16. Phase Contrast Microscopy. Part III: Radioactivity: 17. Radio isotopes in Analytical Techniques. 18. Tracer and Tracer Techniques. 19. Biological Effects of Radiation. 20. Autoradiography. 21. Radiation Dosimetry. Part IV: Statistical Methods: 22. Statistical Methods. Part V: Electrophysiological Methods: 23. Electrophysiological Methods. Part VI: Histochemical and Immunotechniques: 24. Flowcytometry and Cytophotometry. 25. Antibody and Immunochemistry. Part VII: Molecular Biology and Recombinant DNA Methods: 26. Electrophoresis and Electrofocusing. 27. Centrifugation. 28. Nucleic Acid Hybridization and Cot Curves. 29. Polymerase Chain Reaction. 30. Sequencing of Proteins. 31. Nucleic Acid Sequencing. 32. Strategies for Genome Sequencing and Cloning. 33. Polymorphism. 34. Library Generation. 35. Mutagenesis. 36. Carbohydrates. 37. Gene Expression. 38. Microarray Analysis of Gene Expression. 39. Gene and Gene Knock Out. 40. Recombinant Protein Expression. 41. Lipids. Index.
This comprehensive and accessible text discusses all the topics prescribed for the students of Life Sciences taking the National Eligibility Test (NET). Besides, the book would also be useful for undergraduate and postgraduate students of Biotechnology, and postgraduate students of Botany and Zoology.
The book discusses spectroscopy, which forms the core of modern research, be it physical sciences or life sciences, and microscopy, which is now an indispensable analytical tool in Biological Science, with all its different forms. It also illustrates radioactivity and related phenomena so as to justify their widespread applications in modern biological, medical and chemical researches. The book evaluates the role of statistics in biological as well as physiological/medical phenomena, and systematically analyses electrophysiological methods, histochemical and immuno techniques, and molecular biology.
